Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the duodenum, ileum and colon of control, diabetic, and insulin-treated diabetic rats after IL1β-HuC/HuD double-labelling immunohistochemistry. HuC/HuD as a pan-neuronal marker was applied to label myenteric neurons. CD—control duodenum ( a ), CI—control ileum ( b ), CC—control colon ( c ), DD—diabetic duodenum ( d ), DI—diabetic ileum ( e ), DC—diabetic colon ( f ), IDD—insulin-treated diabetic duodenum ( g ), IDI—insulin-treated diabetic ileum ( h ), IDC—insulin-treated diabetic colon ( i ); arrows—IL1β-immunoreactive myenteric neurons, arrowheads—myenteric neurons. Scale bars: 20 μm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control, Immunohistochemistry, Marker

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Proportion of IL1β-immunoreactive myenteric neurons in the duodenum, ileum, and colon of control rats. The proportion of IL1β-immunoreactive myenteric neurons was significantly higher in the colon compared to the ileum and duodenum. Data are expressed as mean ± SEM. **** p < 0.0001 (relative to control duodenum); oooo p < 0.0001 (between control ileum and colon).

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Proportion of IL1β-immunoreactive myenteric neurons of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. In the diabetics, the proportion of IL1β-immunoreactive myenteric neurons was significantly increased in all gut segments, which was prevented by immediate insulin treatment. Data are expressed as mean ± SEM. ** p < 0.01 (relative to controls); o p < 0.05, oooo p < 0.0001 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the colon of control ( a ), diabetic ( b ), and insulin-treated diabetic ( c ) rats after IL1β-nNOS double-labelling immunohistochemistry. CC—control colon, DC—diabetic colon, IDC—insulin-treated diabetic colon; arrows—IL1β-nNOS-immunoreactive myenteric neurons, arrowheads—nNOS-immunoreactive neurons. Scale bars: 20 μm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Proportion of IL1β-nNOS-immunoreactive neurons related to the total number of nNOS-immunoreactive neurons in the myenteric ganglia of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. The proportion of IL1β-nNOS-immunoreactive neurons significantly increased only in the colon of diabetics relative to controls. Data are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 (relative to controls); oo p < 0.01 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Representative fluorescent micrographs of whole-mount preparations of myenteric ganglia from the ileum of control ( a ) and diabetic ( b ) rats after IL1β-CGRP double-labelling immunohistochemistry. CI—control ileum, DI—diabetic ileum; arrows—IL1β-CGRP-immunoreactive myenteric neurons, arrowheads—CGRP-immunoreactive neurons. Scale bars: 20 μm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Proportion of IL1β-CGRP-immunoreactive neurons related to the total number of CGRP-immunoreactive neurons in the myenteric ganglia of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. The proportion of IL1β-CGRP-immunoreactive neurons significantly increased only in the ileum of diabetics relative to controls. Data are expressed as mean ± SEM. ** p < 0.01, **** p < 0.0001 (relative to controls); ooo p < 0.001 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Tissue level of IL1β in intestinal smooth muscle layer homogenates including the myenteric plexus from the colon of control, diabetic and insulin-treated diabetic rats. The IL1β tissue level was significantly increased in diabetics relative to controls, which was prevented by insulin treatment. Data are expressed as means ± SEM. * p < 0.05 (relative to controls). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Representative micrographs of cryosections of myenteric ganglia from the colon of control ( a ), diabetic ( b ) and insulin-treated diabetic ( c ) rats after IL1β RNAscope. IL1β mRNA appear as green punctate dots (arrows), nuclei were counterstained with DAPI (blue). CC—control colon, DC—diabetic colon, IDC—insulin-treated diabetic colon. Scale bars: 20 μm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control, RNAscope

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Representative micrographs of cryosections of mucosa from the duodenum of control ( a ), diabetic ( b ) and insulin-treated diabetic ( c ) rats after IL1β RNAscope. IL1β mRNA appear as green punctate dots (arrows), nuclei were counterstained with DAPI (blue). CD—control duodenum, DD—diabetic duodenum, IDD—insulin-treated diabetic duodenum. Scale bars: 20 μm.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control, RNAscope

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Quantitative evaluation of IL1β mRNA labelling dots in different intestinal layers and gut segments of control rats.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

doi: 10.3390/ijms24065804

Figure Lengend Snippet: Expression of IL1β mRNA in the myenteric ganglia ( a ), smooth muscle ( b ), and intestinal mucosa ( c ) of the duodenum, ileum, and colon of control, diabetic and insulin-treated diabetic rats. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001 (relative to controls); o p < 0.05, oo p < 0.01 (between diabetics and insulin-treated diabetics). C—controls, D—diabetics, ID—insulin-treated diabetics.

Article Snippet: The cryosections were incubated in hydrogen peroxide, followed by protease IV treatment and then hybridized with a rat IL1β probe (Cat. No. 314011-C2, Advanced Cell Diagnostics, Newark, CA, USA; final dilution 1:50) for 2 h at 40 °C.

Techniques: Expressing, Control

Journal: Cells

Article Title: AdhMMP8 Vector Administration in Muscle: An Alternate Strategy to Regress Hepatic Fibrosis

doi: 10.3390/cells12172127

Figure Lengend Snippet: Proinflammatory genes mRNA level expression in liver tissue. A significant decrease in TNF-α and IL-1β mRNA level expression can be observed in the AdhMMP8 vector-treated group at week 7 when compared with the controls (* p < 0.05).

Article Snippet: Taqman probes for COL1A1 (RN00670303_G1), CTGF (RN01537278_G1), TGF-β1 (RN00572010_M1), IL1-β (RN00580432_M1), TNF-α (PN99999017_M1), MMP-1 (LLC185875319), and MMP-9 (RN00579162_M1) rat cDNAs specific detection were bought from Applied Biosystems/Thermo Fisher Scientific (Hammonton, NJ, USA).

Techniques: Expressing, Plasmid Preparation

Journal: Cells

Article Title: AdhMMP8 Vector Administration in Muscle: An Alternate Strategy to Regress Hepatic Fibrosis

doi: 10.3390/cells12172127

Figure Lengend Snippet: Antifibrogenic genes mRNA level expression in liver tissue. A significant increase of MMP-1 and MMP-9 mRNA level expression can be appreciated in the AdhMMP8 vector-treated group at week 6 when compared with the controls (* p < 0.05).

Article Snippet: Taqman probes for COL1A1 (RN00670303_G1), CTGF (RN01537278_G1), TGF-β1 (RN00572010_M1), IL1-β (RN00580432_M1), TNF-α (PN99999017_M1), MMP-1 (LLC185875319), and MMP-9 (RN00579162_M1) rat cDNAs specific detection were bought from Applied Biosystems/Thermo Fisher Scientific (Hammonton, NJ, USA).

Techniques: Expressing, Plasmid Preparation

Journal: Cells

Article Title: AdhMMP8 Vector Administration in Muscle: An Alternate Strategy to Regress Hepatic Fibrosis

doi: 10.3390/cells12172127

Figure Lengend Snippet: Profibrogenic genes mRNA level expression. A significant decrease of COL1A1, TGF-β1, and CTGF mRNA levels is shown in the AdhMMP8 vector-treated group when compared with the controls (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Taqman probes for COL1A1 (RN00670303_G1), CTGF (RN01537278_G1), TGF-β1 (RN00572010_M1), IL1-β (RN00580432_M1), TNF-α (PN99999017_M1), MMP-1 (LLC185875319), and MMP-9 (RN00579162_M1) rat cDNAs specific detection were bought from Applied Biosystems/Thermo Fisher Scientific (Hammonton, NJ, USA).

Techniques: Expressing, Plasmid Preparation

Journal: Cells

Article Title: AdhMMP8 Vector Administration in Muscle: An Alternate Strategy to Regress Hepatic Fibrosis

doi: 10.3390/cells12172127

Figure Lengend Snippet: Profibrogenic genes mRNA level expression. A significant decrease of COL1A1, TGF-β1, and CTGF mRNA levels is shown in the AdhMMP8 vector-treated group when compared with the controls (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Taqman probes for COL1A1 (RN00670303_G1), CTGF (RN01537278_G1), TGF-β1 (RN00572010_M1), IL1-β (RN00580432_M1), TNF-α (PN99999017_M1), MMP-1 (LLC185875319), and MMP-9 (RN00579162_M1) rat cDNAs specific detection were bought from Applied Biosystems/Thermo Fisher Scientific (Hammonton, NJ, USA).

Techniques: Expressing, Plasmid Preparation